(c) Depress ignition switch to light the flame and slowly increase the fuel flow rate. (b) Set the sodium filter in position before the photocell and set the air pressure to the Respectively in distilled water (25mL each), using the 50ug/mL standard sodium
#Sodium emission spectrum series
(a) Prepare a calibration series containing 1.25, 2.5, 5.0 and 7.5ug/mL sodium (A) Optimization of the fuel/air flow rates for determination of sodium: Therefore, addition of radiation buffers is not required for potassium analyses with this instrument. However this effect is negligible in the air/propane flame used by the flame analyser in this experiment. Potassium determination by flame emission is affected mainly by ionization of potassium at the high temperatures associated with air/acetylene or hotter flames, especially at low concentrations of the elements. The addition of a sufficient quantity of aluminium ions to the analyte solution tends to reduce the emission due to calcium and hence minimize the interference (2, 3). In the analysis of sodium and potassium in the presence of calcium, some interference by the latter occurs, due to spectral overlap. The intensity of the isolated radiation is measured by a photosensitive detector coupled to an amplifier and recorder. The required spectral line or a portion of the spectrum is isolated either by a monochromator or by an optical filter. Measurement of the intensity of a portion of a spectrum characteristic of an element can provides a measure of the concentration in the sample. The spectrum may be relatively simple, consisting of only a few lines, or may be complex, broad bands. propane/air, acetylene/air) which is hot enough to cause the element to allow emission of characteristic radiation. The method has also been applied, with varying degree of success, to determine of perhaps half the elements in the periodic table.įlame photometry is based on the emission spectrum of an element which is excited in a flame (e.g. For reasons of convenience, speed, and relative freedom from interferences, flame emission spectroscopy has become the method of choice for these otherwise difficult to determine elements. Its most important uses have been in the determination of sodium, potassium, lithium and calcium, particularly in biological fluids and tissues. Investigation of some factors affecting accuracy of sodium determinationĪnd their application in the determination of sodiumĪtomic emission spectroscopy (AES) employing flames, also called flame emission spectroscopy (FES) or flame photometry has found widespread application in elemental analysis (1). To get the labguide for this experiment click here Enjoy! Includes a helpful analytical notes feature. Preparations, chemicals, apparatus and sample requirements. A labguide has been produced for each experiment.
0 kommentar(er)
